Validating microarray data using rt real time pcr rabbi wolpe dating

The technique relies on the relative quantification of m RNA expression in cells or tissues.

Circulating blood cells can be used as an alternative to tissue biopsies when these are not available.

Both platforms consistently underestimate the relative changes in m RNA expression between experimental and control samples.

The bias observed with c DNA arrays was predictable for fold-changes We describe detailed protocols and results with an integrated platform for studying relative transcript expression, including microarray design and fabrication, analysis and calibration algorithms, and high throughput quantitative real-time PCR.

This approach optimizes sensitivity and accuracy while controlling the cost of experiments.

A high quality c DNA array was fabricated using a restricted number of carefully selected transcripts with each clone printed in triplicate.

validating microarray data using rt real time pcr-73validating microarray data using rt real time pcr-77

Recently Added201720162015201420132012201120102000-2009 Alevizakos M, Gaitanidis A, Nasioudis D, et al. Use the article search box above, or try advanced search form.Need information about the journal or about submitting a manuscript? Please use the feedback form and provide a detailed description of the problem.Overall, significant correlation was observed between microarray and q PCR results (ρ=0.708, p), array averaging, tissue type, and tissue preparation was assessed.Filtering of microarray data for measures of quality (fold-change and ρ-value) proves to be the most critical factor, with significant correlations of ρτ;0.80 consistently observed when quality scores are applied.